|
KMID : 1134820170460020169
|
|
Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 2 p.169 ~ p.176
|
|
|
Effect of Tartary Buckwheat Sprout on Non-Alcoholic Fatty Liver Disease through Anti-Histone Acetyltransferase Activity
|
|
Hwang Jin-Taek
Nam Tae-Gyu
Chung Min-Yu
Park Jae-Ho
Choi Hyo-Kyoung
|
|
|
Abstract
|
|
|
|
Non-alcoholic fatty liver disease (NAFLD) is caused by chronic lipid accumulation due to dysregulation of lipid metabolism in the liver, and it is associated with various human diseases such as obesity, dyslipidemia, hypertension, and diabetes. Histone acetylation is a representative epigenetic mechanism regulated by histone acetyltransferases (HATs) and deacetylases. We observed that tartary buckwheat sprout (TBS) suppressed lipid accumulation in HepG2 cells through its anti-HAT activity. We showed that TBS was a novel HAT inhibitor with specificity for the major HAT enzyme p300. Importantly, TBS reduced acetylation of total and histone proteins, H3K9, H3K36, and H4K8, resulting in decreased transcriptional activities of sterol regulatory element-binding protein 1c, ATP citrate lyase, and fatty acid synthase. These results suggest that TBS inhibits the NAFLD transcription-modulating activity of lipogenesis-related genes through modification of histone acetylation.
|
|
|
KEYWORD
|
|
|
non-alcoholic fatty liver disease, histone acetyltransferase, lipid accumulation, HepG2, tartary buckwheat sprout
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|